Bioinformatic Methods I

Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. However, the challenge facing scientists is analyzing and even accessing these data to extract useful information pertaining to the system being studied. This course focuses on employing existing bioinformatic resources – mainly web-based programs and databases – to access the wealth of data to answer questions relevant to the average biologist, and is highly hands-on. Topics covered include multiple sequence alignments, phylogenetics, gene expression data analysis, and protein interaction networks, in two separate parts. The first part, Bioinformatic Methods I (this one), deals with databases, Blast, multiple sequence alignments, phylogenetics, selection analysis and metagenomics. The second part, Bioinformatic Methods II, covers motif searching, protein-protein interactions, structural bioinformatics, gene expression data analysis, and cis-element predictions. This pair of courses is useful to any student considering graduate school in the biological sciences, as well as students considering molecular medicine. Both provide an overview of the many different bioinformatic tools that are out there. These courses are based on one taught at the University of Toronto to upper-level undergraduates who have some understanding of basic molecular biology. If you're not familiar with this, something like BIO101 from Saylor Academy (https://learn.saylor.org/course/view.php?id=889) might be helpful. No programming is required for this course. Bioinformatic Methods I is regularly updated, and was completely updated for January 2026

• Understand use of bioinformatics databases to access large biological datasets
• Apply Blast tool for genetic sequence analysis
• Perform multiple sequence alignment to study evolutionary differences
• Analyze phylogenetic classification to understand evolutionary relationships
• Explore metagenomic techniques for microbial community analysis

• Basic computer and internet skills
• Ability to read and understand course instructions in English
• Willingness to complete short practical exercises